The Duchess of Sussex has complained to Ofcom about Piers Morgan's comments about her on Good Morning Britain, the UK broadcast regulator has confirmed.
On the ITV breakfast show on Monday, Morgan said he "didn't believe a word" Meghan had told interviewer Oprah Winfrey about life in the Royal Family.
The duchess spoke about having suicidal thoughts, and that she got no support.
More than 41,000 others have also complained to Ofcom about Morgan, who left the show the following day.
An Ofcom spokesperson said: "We can confirm receipt of a standards complaint made on behalf of the Duchess of Sussex."
The duchess is also reported to have complained to ITV directly.
"Who did you go to?" he said. "What did they say to you? I'm sorry, I don't believe a word she said, Meghan Markle. I wouldn't believe it if she read me a weather report.
"The fact that she's fired up this onslaught against our Royal Family I think is contemptible."
On Tuesday's episode, Morgan clashed with weather presenter Alex Beresford during a discussion about the interview, which prompted Morgan to walk out of the studio.
His permanent departure from the programme was announced that evening. Morgan stood by his comments the following day, saying: "Freedom of speech is a hill I'm happy to die on."
It was also announced on Friday that the duke and duchess have donated to a new charity that aims to help young people from diverse backgrounds get into journalism.
The Duke and Duchess's Archewell Foundation are among the first donors, but it is not known how much money they have contributed.
PressPad founder Olivia Crellin told BBC News the charity had been approached by the duke and duchess's team "very recently".
"They just wanted to give a gift as a part of a couple of organisations that they are donating to," she said. "They felt that our mission and our values aligned with theirs."
Prince Harry told Oprah the UK tabloid press is "bigoted" and creates a "toxic environment" of "control and fear".
The couple's interview has sparked scrutiny of the media and the role race has played in its coverage of Meghan.